Many users ask us about how to download data from ensembl. Usually, the answer is using BioMart. Comparative genomics data are also available in the standard Mart for your favorite species. For instance to get all the human-mouse orthologs, one can select the human dataset, filter all the genes with no mouse orthologs and choose to output the mouse orthologs for all the resulting genes.
Here is how to get these data in 10 simple steps
1. Go to: http://www.ensembl.org/biomart/martview
2. Choose “Ensembl 52”
3. Choose “Homo sapiens genes (NCBI36)”
4. Click on “Filters” in the left menu
5. Unfold the “MULTI SPECIES COMPARISONS” box, tick the “Homolog filters” option and choose “Orthologous Mouse Genes” from the drop-down menu.
6. Click on “Attributes” in the left menu
7. Click on “Homologs”
8. Unfold the “MOUSE ORTHOLOGS” box and select the data you want to get (most probably the gene ID and maybe the orthology type as well).
9. Click on the “Results” button (top left)
10. Choose your favorite output
Here is the preview of the results: